Faculty Directory
Keith E.J. Tyo

Associate Professor of Chemical and Biological Engineering

Contact

2145 Sheridan Road
Tech E156
Evanston, IL 60208-3109

847-467-2972Email Keith Tyo

Website

Tyo Research Group


Centers

Center for Synthetic Biology


Departments

Chemical and Biological Engineering

Affiliations

PhD Program in Interdisciplinary Biological Sciences


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Education

Ph.D., MIT, Cambridge, MA

M.S., MIT, Cambridge, MA

B.S., West Virginia University, Morgantown, WV

 


Research Interests

Synthetic biology, metabolic engineering, global health

What we do

Microbes must cope with harsh, rapidly changing environments to survive. To do this, microbes have developed sophisticated mechanisms to (a) sense the changes in the environment, and (b) respond quickly to these changes to protect itself from harm or capitalize on an opportunity. Our lab seeks to rewire these fundamental input/output relationships to program cells to do useful things for mankind in a paradigm called synthetic biology. Inputs: We study methods to modify existing environmental detection sensors in yeast and modify them to detect new analytes. Outputs: We investigate ways microbes modify their metabolic networks and use these modifications to increase production of a given metabolite.


Why we do it

Synthetic biology offers a disruptive technology that has vast potential to impact the most important facets of our lives. The way we diagnose diseases, the drugs we use, the fuels we put in our cars, the plastic that is used in our potato chip bag - we have the opportunity to improve the sustainability of our lifestyle. And by reducing costs, we can make aspects of our lifestyle available to those who could not otherwise afford it. We hope to offer technical contributions that, simply put, make the world a better place.


Selected Publications

  • Fackler, Nick; Heijstra, Bjodiern D.; Rasor, Blake J.; Brown, Hunter; Martin, Jacob; Ni, Zhuofu; Shebek, Kevin M.; Rosin, Rick R.; Simpson, Seacutean D.; Tyo, Keith E.; Giannone, Richard J.; Hettich, Robert L.; Tschaplinski, Timothy J.; Leang, Ching; Brown, Steven D.; Jewett, Michael C.; Kodiepke, Michael, Stepping on the Gas to a Circular Economy, Annual Review of Chemical and Biomolecular Engineering 12:439-470.
  • Glasscock, Cameron J.; Biggs, Bradley W.; Lazar, John T.; Arnold, Jack H.; Burdette, Lisa A.; Valdes, Aliki; Kang, Min Kyoung; Tullman-Ercek, Danielle; Tyo, Keith E.J.; Lucks, Julius B., Dynamic Control of Gene Expression with Riboregulated Switchable Feedback Promoters, ACS synthetic biology 10(5):1199-1213.
  • Ni, Zhuofu; Stine, Andrew E.; Tyo, Keith E.J.; Broadbelt, Linda J., Curating a comprehensive set of enzymatic reaction rules for efficient novel biosynthetic pathway design, Metabolic Engineering 65:79-87.
  • Biggs, Bradley W.; Bedore, Stacy R.; Arvay, Erika; Huang, Shu; Subramanian, Harshith; McIntyre, Emily A.; Duscent-Maitland, Chantel V.; Neidle, Ellen L.; Tyo, Keith E.J., Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1, Nucleic acids research 48(9):5169-5182.
  • Love, Aaron M.; Biggs, Bradley W.; Tyo, Keith E.J.; Ajikumar, Parayil Kumaran, Chemically inducible chromosomal evolution (CIChE) for multicopy metabolic pathway engineering, Humana Press Inc.:37-45.
  • John, Peter C.St; Strutz, Jonathan; Broadbelt, Linda J.; Tyo, Keith E.J.; Bomble, Yannick J., Bayesian inference of metabolic kinetics from genome-scale multiomics data, PLoS computational biology 15(11).
  • Stainbrook, Sarah C.; Tyo, Keith E.J., Model-guided mechanism discovery and parameter selection for directed evolution, Applied Microbiology and Biotechnology 103(23-24):9697-9709.
  • Strutz, Jonathan; Martin, Jacob; Greene, Jennifer; Broadbelt, Linda; Tyo, Keith, Metabolic kinetic modeling provides insight into complex biological questions, but hurdles remain, Current Opinion in Biotechnology 59:24-30.