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  • May
    8

    EES Seminar- Mapping Early Epigenetic and Transcriptomic Responses to Heat Stress in Reef-Building Coral Using Novel ChIP-seq Methodology- George Warfel

    McCormick - Civil and Environmental Engineering (CEE)

    2:00 PM A230, Technological Institute

    EVENT DETAILS

    Abstract: Coral reefs face significant challenges under rapid environmental change, driven by human activity throughout the last century. Steadily increasing sea surface temperatures, in conjunction with dramatic marine heat waves, induce an environmental stress response in corals that often leads to bleaching and death. During heat stress, corals must respond rapidly at the cellular level to regulate molecular processes and improve their chances of survival. Previous studies have characterized several transcriptomic shifts associated with the coral heat stress response. However, the upstream epigenetic mechanisms that regulate gene expression remain largely unknown in these organisms. To address this, we focused on chromatin packing, a process that controls the accessibility of genes to transcriptional machinery via chemical modifications of histone proteins. To study these mechanisms, we adapted a Chromatin Immunoprecipitation sequencing (ChIP-seq) protocol for reef-building corals. This technique uses immunoprecipitation to map enrichment of histone post-translational modifications across the genome through sequencing. After verifying the robustness of this protocol in coral tissues, we deployed ChIP-seq during an acute 24-hour heat stress assay to measure early epigenetic responses in the coral Pocillopora damicornis. To confirm whether specific histone modifications resulted in concomitant shifts in gene expression, we performed mRNA-sequencing at identical time points. We identified significant changes in the coral epigenome as early as ~3 hours after initiating heat stress, with differences increasing in magnitude under prolonged exposure. Notably, we observed significant increases in H3K27 acetylation—a marker associated with active transcription—on genes involved in protein misfolding, immune responses, cell signaling, and transcriptional regulation. In parallel, a loss of H3K27 acetylation was observed in genes related to transmembrane transport and metabolic processes. These findings are consistent with the cellular stress responses outlined in previous literature and provide novel mechanistic insight into how corals rapidly regulate their survival at the epigenetic level. To our knowledge, this study represents the first locus-specific epigenetic profiling of corals during heat stress.

    Biosketch: George Warfel is a fourth-year undergraduate at Northwestern University studying Chemical Engineering and Biological Sciences. He works in the Marcelino lab within the Civil and Environmental Engineering department and Center for Physical Genomics and Engineering. His research focuses on probing the molecular mechanisms that drive environmental stress responses in reef-building corals using next-generation sequencing techniques. He was recently awarded the NSF Graduate Research Fellowship and plans to continue his studies at a PhD program in bioengineering or molecular sciences.

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    TIME Friday, May 8, 2026 at 2:00 PM - 3:00 PM

    LOCATION A230, Technological Institute    map it

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    CONTACT Andrew Liguori    andrew.liguori@northwestern.edu EMAIL

    CALENDAR McCormick - Civil and Environmental Engineering (CEE)

  • May
    21

    CAE Seminar- Traffic-Related Air Pollution, Extreme Weather, and Green Space: Advancing Engineering–Public Health Integration- Kai Zhang

    McCormick - Civil and Environmental Engineering (CEE)

    4:00 PM Transportation Center, Chambers Hall

    EVENT DETAILS

    TIME Thursday, May 21, 2026 at 4:00 PM - 5:00 PM

    LOCATION Transportation Center, Chambers Hall    map it

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    CONTACT Andrew Liguori    andrew.liguori@northwestern.edu EMAIL

    CALENDAR McCormick - Civil and Environmental Engineering (CEE)

  • May
    22

    EES Seminar- How Coral DNA is Packed in 3D and Why It May Matter for Stress Relief- Shanna Davidson

    McCormick - Civil and Environmental Engineering (CEE)

    2:00 PM A230, Technological Institute

    EVENT DETAILS

    TIME Friday, May 22, 2026 at 2:00 PM - 3:00 PM

    LOCATION A230, Technological Institute    map it

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    CONTACT Andrew Liguori    andrew.liguori@northwestern.edu EMAIL

    CALENDAR McCormick - Civil and Environmental Engineering (CEE)

  • May
    27

    CAE Seminar- The Mechanics of Natural Hazards: From Ice Sheets to Debris Flows- Victor Tsai

    McCormick - Civil and Environmental Engineering (CEE)

    11:00 AM A230, Technological Institute

    EVENT DETAILS

    TIME Wednesday, May 27, 2026 at 11:00 AM - 12:00 PM

    LOCATION A230, Technological Institute    map it

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    CONTACT Andrew Liguori    andrew.liguori@northwestern.edu EMAIL

    CALENDAR McCormick - Civil and Environmental Engineering (CEE)

  • May
    29

    EES Seminar- Anaerobic Biotechnology: How Much Acetate can a Methanogen Eat in a Day and Why is it Important?- Dan Zitomer

    McCormick - Civil and Environmental Engineering (CEE)

    2:00 PM A230, Technological Institute

    EVENT DETAILS

    TIME Friday, May 29, 2026 at 2:00 PM - 3:00 PM

    LOCATION A230, Technological Institute    map it

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    CONTACT Andrew Liguori    andrew.liguori@northwestern.edu EMAIL

    CALENDAR McCormick - Civil and Environmental Engineering (CEE)

  • Jun
    11

    Undergraduate Cocktail Reception

    McCormick - Civil and Environmental Engineering (CEE)

    6:30 PM 1026 Davis St Upstairs

    EVENT DETAILS

    TIME Thursday, June 11, 2026 at 6:30 PM - 9:30 PM

    LOCATION 1026 Davis St Upstairs   

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    CONTACT Andrew Liguori    andrew.liguori@northwestern.edu EMAIL

    CALENDAR McCormick - Civil and Environmental Engineering (CEE)

  • Jun
    12

    CEE Graduation and Recognition Ceremony

    McCormick - Civil and Environmental Engineering (CEE)

    2:00 PM 1702 Chicago Ave

    EVENT DETAILS

    TIME Friday, June 12, 2026 at 2:00 PM - 4:00 PM

    LOCATION 1702 Chicago Ave   

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    CONTACT Andrew Liguori    andrew.liguori@northwestern.edu EMAIL

    CALENDAR McCormick - Civil and Environmental Engineering (CEE)

  • Sep
    16

    CEE Faculty Retreat

    McCormick - Civil and Environmental Engineering (CEE)

    10:00 AM 1702 Chicago Ave

    EVENT DETAILS

    TIME Wednesday, September 16, 2026 at 10:00 AM - 4:00 PM

    LOCATION 1702 Chicago Ave   

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    CONTACT Andrew Liguori    andrew.liguori@northwestern.edu EMAIL

    CALENDAR McCormick - Civil and Environmental Engineering (CEE)

  • Sep
    16

    CEE Department BBQ

    McCormick - Civil and Environmental Engineering (CEE)

    4:00 PM

    EVENT DETAILS

    TIME Wednesday, September 16, 2026 at 4:00 PM - 7:00 PM

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    CONTACT Andrew Liguori    andrew.liguori@northwestern.edu EMAIL

    CALENDAR McCormick - Civil and Environmental Engineering (CEE)

  • Oct
    3

    CEE Fall Career Fair

    McCormick - Civil and Environmental Engineering (CEE)

    9:00 AM

    EVENT DETAILS

    TIME Saturday, October 3, 2026 at 9:00 AM - 12:00 PM

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    CONTACT Andrew Liguori    andrew.liguori@northwestern.edu EMAIL

    CALENDAR McCormick - Civil and Environmental Engineering (CEE)