Faculty Directory
William M. Miller

Professor of Chemical and Biological Engineering

Director, Master of Science in Biotechnology Program

Contact

2145 Sheridan Road
Tech E248
Evanston, IL 60208-3109

847-491-4828Email William Miller

Website

Miller Research Group

Master of Biotechnology


Departments

Chemical and Biological Engineering

Affiliations

Master of Biotechnology Program


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Education

Ph.D. Chemical Engineering, University of California, Berkeley, CA

S.M. Chemical Engineering, MIT, Cambridge, MA

B.S. Chemical Engineering (Hons), Lehigh University, Bethlehem, PA


Research Interests

Human and animal cell culture applications in biotechnology, medicine, and tissue engineering. 

Ex vivo expansion and controlled differentiation of hematopoietic (blood) stem and progenitor cells for transplantation therapies, with a focus on megakaryocytic cells and platelets.  Development of culture surfaces and microbioreactors that mimic aspects of the in vivo bone marrow niche to increase megakaryocytic differentiation and stimulate platelet release.  Analysis of mechanisms regulating hematopoietic differentiation, including epigenetics, protein acetylation, and transcription factor activity networks. Development of bioreactor systems for hematopoietic cell and platelet production. 

Development and characterization of bioreactor systems for the recellularization of organ scaffolds for eventual use in liver and kidney transplantation (with Prof. Jason Wertheim in Transplant Surgery).

Environmental aspects of water, energy, and sustainability.

Evaluation of ecosystem services provided by urban nature preserves and green infrastructure including water storage, flood mitigation, urban microclimate regulation, and air pollution mitigation. We are collaborating with Argonne researchers Cristina Negri and Pete Beckman in a joint project with Aaron Packman (Civil and Environmental Engineering) at Northwestern to instrument, monitor, and model ecosystem services provided by the Indian Boundary Prairies (high-quality remnant prairies owned by The Nature Conservancy and Northeastern Illinois University) in Markham, IL.


Selected Publications

    "Energy, Water and Fish: Biodiversity Impacts of Energy-Sector Water Demand in the United States Depend on Efficiency and Policy Measures," R.I. McDonald, J.D. Olden, J.J. Opperman, W.M. Miller, J. Fargione, C. Revenga; J.V. Higgins, and J. Powell, PLoS ONE, 7: e50219 (17 pages) (2012).

    "Bioreactor design for perfusion-based, highly-vascularized organ regeneration," B.M. Bijonowski, W.M. Miller*, and J.A. Wertheim*, Curr. Opin. Chem. Eng., 2: 32–40 (2013). PMCID: PMC3610919.

    "Three-Stage Ex Vivo Expansion of High-Ploidy Megakaryocytic Cells: Towards Large-Scale Platelet Production," S. Panuganti, A.C. Schlinker, P.F. Lindholm, E.T. Papoutsakis, and W.M. Miller, Tissue Eng. A, 19: 998-1014 (2013).

    "Profiling Deacetylase Activities in Cell Lysates with Peptide Arrays and SAMDI Mass Spectrometry," H.-Y. Kuo, T.A. DeLuca, W.M. Miller*, and M. Mrksich*, Anal. Chem., 85: 10635-10642 (2013) (Editors' Highlight).

    "Dynamic transcription factor activity profiles reveal key regulatory interactions during megakaryocyte and erythrocyte differentiation," M.T. Duncan, S. Shin, J.J. Wu, Z. Mays S. Weng, N. Bagheri*, W.M. Miller*, L.D. Shea*, Biotechnol. Bioeng. 111: 2082-2094(2014).

    "Human Megakaryocyte Progenitors Derived from Hematopoietic Stem Cells of Normal Individuals are MHC class II-Expressing Professional APC that Enhance Th17 and Th1/Th17 Responses," A. Finkielsztein, A.C. Schlinker, L. Zhang, W.M. Miller, S.K. Datta, Immunol. Lett. 163:84-95 (2015).

    "Optimization and critical evaluation of decellularization strategies to develop renal extracellular matrix scaffolds as biological templates for organ engineering and transplantation," M. Caralt, J.S. Uzarski, S. Iacob, K.P. Obergfell, N. Berg, B.M. Bijonowski, K.M. Kiefer, H.H. Ward, A. Wandinger-Ness, W.M. Miller, Z.J. Zhang, M.M. Abecassis, J.A. Wertheim, Am. J. Transplantation 15:64-75 (2015).

    "Separation of in-vitro-derived megakaryocytes and platelets using spinning-membrane filtration," A.C. Schlinker, K. Radwanski, C. Wegener, K. Min*, W.M. Miller*, Biotechnol. Bioeng. 112:788-800(2015).

    "Dual-purpose bioreactors for recellularization and maintenance culture of bioengineered kidney and liver scaffolds," J.S. Uzarski, B.M. Bijonowski, B. Wang, H.H. Ward, A. Wandinger-Ness, W.M. Miller*, J.A. Wertheim*, Tissue Engineering Part C. 21:1032-1043 (2015).

    "HOXA10 null animals exhibit reduced platelet biogenesis," I.M. Konieczna, T.A. DeLuca, E.A. Eklund, W.M. Miller, Br. J. Haematol., 173:303-313 (2016).

    "Megakaryocyte polyploidization and proplatelet formation in low-attachment conditions," A.C. Schlinker, M.T. Duncan, T.A. DeLuca, D.C. Whitehead, W.M. Miller, Biochem. Eng. J., 111:24-33 (2016).

    "SIRT1 is a critical regulator of K562 cell growth, survival, and differentiation," M.T. Duncan, T.A. DeLuca, H.-Y. Kuo, M. Yi, M. Mrksich, W.M. Miller, Exp. Cell Res., 344:40–52 (2016).