Faculty Directory
William M. Miller

Professor of Chemical and Biological Engineering

Director, Master of Science in Biotechnology Program

Contact

2145 Sheridan Road
Tech E248
Evanston, IL 60208-3109

847-491-4828Email William Miller

Website

Miller Research Group

Master of Biotechnology


Departments

Chemical and Biological Engineering

Affiliations

Master of Biotechnology Program


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Education

Ph.D. Chemical Engineering, University of California, Berkeley, CA

S.M. Chemical Engineering, MIT, Cambridge, MA

B.S. Chemical Engineering (Hons), Lehigh University, Bethlehem, PA


Research Interests

Human and animal cell culture applications in biotechnology, medicine, and tissue engineering. 

Ex vivo expansion and controlled differentiation of hematopoietic (blood) stem and progenitor cells for transplantation therapies, with a focus on megakaryocytic cells and platelets.  Development of culture surfaces and microbioreactors that mimic aspects of the in vivo bone marrow niche to increase megakaryocytic differentiation and stimulate platelet release.  Analysis of mechanisms regulating hematopoietic differentiation, including epigenetics, protein acetylation, and transcription factor activity networks. Development of bioreactor systems for hematopoietic cell and platelet production. 

Development and characterization of bioreactor systems for the recellularization of organ scaffolds for eventual use in liver and kidney transplantation (with Prof. Jason Wertheim in Transplant Surgery).

Environmental aspects of water, energy, and sustainability.

Environmental aspects of water, energy, and sustainability with a focus on ecosystem services provided by urban nature preserves and green infrastructure (GI) including water storage, flood mitigation, urban microclimate regulation, and air pollution mitigation. Data from a network of groundwater, surface water, and soil moisture sensors provides information on water storage and transport across Gensburg-Markham Prairie (GMP a high quality remnant prairie owned by The Nature Conservancy and Northeastern Illinois University) as a function of precipitation history, season, and time for use in predicting GMP water storage and release dynamics to understand the impact for mitigating flooding in surrounding neighborhoods. Research in Chatham, the Chicago neighborhood with the most flood insurance claims in 2007-2011, focuses on the impacts of installing GI, such as rain gardens and dry wells, in urban neighborhoods. (with Prof. Aaron Packman in Civi and Environmental Engineering)


Selected Publications

    "Energy Sprawl or Energy Efficiency: Climate Policy Impacts on Natural Habitat for the United States of America," R.I. McDonald, J. Fargione, J. Kiesecker, W.M. Miller, and J. Powell, PLoS ONE, 4: e6802 (11 pages) (2009).

    "Energy, Water and Fish: Biodiversity Impacts of Energy-Sector Water Demand in the United States Depend on Efficiency and Policy Measures," R.I. McDonald, J.D. Olden, J.J. Opperman, W.M. Miller, J. Fargione, C. Revenga; J.V. Higgins, and J. Powell, PLoS ONE, 7: e50219 (17 pages) (2012).

    "Three-Stage Ex Vivo Expansion of High-Ploidy Megakaryocytic Cells: Towards Large-Scale Platelet Production," S. Panuganti, A.C. Schlinker, P.F. Lindholm, E.T. Papoutsakis, and W.M. Miller, Tissue Eng. A, 19: 998-1014 (2013).

    "Profiling Deacetylase Activities in Cell Lysates with Peptide Arrays and SAMDI Mass Spectrometry," H.-Y. Kuo, T.A. DeLuca, W.M. Miller*, and M. Mrksich*, Anal. Chem., 85: 10635-10642 (2013) (Editors' Highlight).

    "Dynamic transcription factor activity profiles reveal key regulatory interactions during megakaryocyte and erythrocyte differentiation," M.T. Duncan, S. Shin, J.J. Wu, Z. Mays S. Weng, N. Bagheri*, W.M. Miller*, L.D. Shea*, Biotechnol. Bioeng. 111: 2082-2094(2014).

    "Optimization and critical evaluation of decellularization strategies to develop renal extracellular matrix scaffolds as biological templates for organ engineering and transplantation," M. Caralt, J.S. Uzarski, S. Iacob, K.P. Obergfell, N. Berg, B.M. Bijonowski, K.M. Kiefer, H.H. Ward, A. Wandinger-Ness, W.M. Miller, Z.J. Zhang, M.M. Abecassis, J.A. Wertheim, Am. J. Transplantation 15:64-75 (2015).

    "Separation of in-vitro-derived megakaryocytes and platelets using spinning-membrane filtration," A.C. Schlinker, K. Radwanski, C. Wegener, K. Min*, W.M. Miller*, Biotechnol. Bioeng. 112:788-800(2015).

    "Dual-purpose bioreactors for recellularization and maintenance culture of bioengineered kidney and liver scaffolds," J.S. Uzarski, B.M. Bijonowski, B. Wang, H.H. Ward, A. Wandinger-Ness, W.M. Miller*, J.A. Wertheim*, Tissue Engineering Part C. 21:1032-1043 (2015).

    "Megakaryocyte polyploidization and proplatelet formation in low-attachment conditions," A.C. Schlinker, M.T. Duncan, T.A. DeLuca, D.C. Whitehead, W.M. Miller, Biochem. Eng. J., 111:24-33 (2016).

    "SIRT1 is a critical regulator of K562 cell growth, survival, and differentiation," M.T. Duncan, T.A. DeLuca, H.-Y. Kuo, M. Yi, M. Mrksich, W.M. Miller, Exp. Cell Res., 344:40–52 (2016).

    "Essential design considerations for using the resazurin reduction assay to noninvasively quantify cell expansion within perfused extracellular matrix scaffolds," J.S. Uzarski, M.D. DiVito, J.A. Wertheim*, W.M. Miller*, Biomaterials, 129:163-175 (2017).

    “A uniform-shear rate microfluidic bioreactor for real-time study of proplatelet formation and rapidly-released platelets,” A.F. Martinez, R.D. MacMahon, M. Horner, W.M. Miller, Biotechnology Progress33:1614-1629 (2017) (cover image).