BME 333: Modern Optical Microscopy and Imaging

Quarter Offered

Fall : TTh 9:30-10:50 ; Zhang


PHYSICS 135-2, MATH 220, MATH 230, GEN ENG 205-4


This course provides students with (1) Fundamental background of tissue optics; (2) Understanding of physics, strengths, and limitations of various existing bio-optical imaging technologies; (3) Knowledge of emerging bio-optical imaging technologies for anatomic and functional studies.

Who takes it?

Senior undergraduate students and graduate students.


  • Introduction to optics, optical properties of tissue, and photon-tissue interactions
  • Monte Carlo simulation
  • Sensing of optical properties and spectroscopy
  • Ballistic imaging
  • Wide-field and dark-field microscopy
  • Polarization, phase contrast, and differential interference contract microscopy (DIC) microscopy
  • Fluorescence microscopy
  • Confocal microscopy
  • Two-photon microscopy
  • Optical coherence tomography
  • Super-resolution imaging

Required Class Materials

Fundamentals of Light Microscopy and Electronic Imaging, Douglas B. Murphy, Wiley-Liss, ISBN: 0-471-25391-X

Suggested Class Materials

Biomedical Optics: Principles and Imaging, Lihong V. Wang and Hsin-i Wu, Wiley Interscience, 1st Edition, ISBN: 0471743046