Faculty Directory
Michael Jewett

Walter P. Murphy Professor of Chemical and Biological Engineering


2145 Sheridan Road
Silverman 3621 & 1710
Evanston, IL 60208-3109

847-467-5007Email Michael Jewett


Jewett Lab


Center for Synthetic Biology


Chemical and Biological Engineering


PhD Program in Interdisciplinary Biological Sciences


Ph.D. Chemical Engineering, Stanford University, Palo Alto, CA

M.S. Chemical Engineering, Stanford University, Palo Alto, CA

B.S. Chemical Engineering, Bioengineering specialization (summa cum laude), University of California, Los Angeles, CA

Research Interests

Cell-free synthetic biology, protein synthesis, therapeutics, glycosylation, engineered ribosomes

Our research group is motivated by a desire to understand, harness, and expand the capabilities of biological systems for compelling applications in medicine, materials, and energy. Specifically, we focus on designing, constructing, and modifying biological systems involved in protein synthesis and metabolism to (i) understand why nature’s designs work the way they do and (ii) open the way to products that have been impractical, if not impossible, to produce by other means. An innovative feature of our research program is the use of cell-free systems. The foundational principle is that we can conduct precise, complex biomolecular transformations without using intact cells, which provides an unprecedented and otherwise unattainable freedom of design to modify and control biological systems. For example, cell-free systems avoid the need to balance the tug-of-war that exists between the cell’s physiological and evolutionary objectives and the engineer’s process objectives. We are also pioneering new directions to repurpose the translation apparatus for synthetic biology. The goal is to monitor, interrogate, and understand the process of translation, and with this knowledge diversify, evolve and repurpose the ribosome and its peripheral machinery into a re-engineered machine to generate non-natural polymers as new classes of sequence-defined, evolvable matter. 

Our research activities are structured into four thrust areas: (1) cell-free protein synthesis and orthogonal translation systems, (2) engineered ribosomes, (3) metabolic engineering, and (4) glycosylation. These research areas are connected by approach (e.g., cell-free systems) and their focus on biocatalytic systems (e.g., the translation apparatus). They advance new understanding of biological knowledge and develop enabling technologies for the production of therapeutics, biomaterials, and biochemicals. 

Selected Publications

    Silverman, A.D., Karim, A.S., and Jewett, M.C. 2019. Cell-free gene expression systems: An expanding repertoire of applications. Nature Reviews Genetics. 

    Jaroentomeechai, T., Stark, J.C., Natarajan, A., Glasscock, C., Yates, L., Hsu, K.J., Mrksich, M., Jewett, M.C., and DeLisa, M.P. 2018. Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery. Nature Communications. 9, 2686.  

    Kightlinger, W.,  Lin, L., Rosztoczy, M., DeLisa, M.P., Mrksich, M., and Jewett, M.C.  2018. Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferase. Nature Chemical Biology. 14, 627-635

    Kightlinger, W., Duncker, K., Ramesh, A., Thames, A., Natarajan, A., Stark, J.C., Yang, A., Lin, L., Mrksich, M., DeLisa, M.P., and Jewett, M.C. 2019. A. cell-free biosynthesis platform for modular construction of protein glycosylation pathways. Nature Communications. 10: 5404.

    Martin, R.W., Des Soye, B.J., Kwon, Y.C., Kay, J., Davis, R.G., Thomas, P.M., Marcum, R.D., Majewska, N.I., Chen, C.X., Weiss, M.G., Stoddart, A.E., Amiram, M., Charna, A.K., Isaacs, F.J., Kelleher, N.L., Hong, S.H., and Jewett, M.C. 2018. Cell-free protein synthesis from genomically recoded bacteria enables multi-site incorporation of non-canonical amino acids. Nature Communications. 9, 1203.

    Stark, J.C., Huang, A., Nguyen, P.Q., Dubner, R.S., Hsu, K.J., Ferrante,T., Anderson, M., Kanapskyte, A., Mucha, Q., Packett, J.S., Patel, P., Patel, R., Qaq, D., Zondor, T., Burke, J., Martinez, T., Miller-Berry, A., Puppala, A., Reichert, K., Schmid, M., Brand, L., Hill, L.R., Chellaswamy, J.F., Faheem, N., Fetherling, S., Gong, E., Gonzalzles, E.M., Granito, T., Koritsaris, J., Nguyen, B., Ottman, S., Palffy, C., Patel, A., Skweres, S., Slaton, A., Woods, T., Donghia, N., Pardee, K., Collins, J.J., Jewett, M.C. 2018. BioBits Bright: a fluorescent synthetic biology education kit. Science Advances. EAAT5107. 

    Karim, A.S and Jewett, M.C. 2016. A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery. Metabolic Engineering. 36:116-126.  

    Orelle C., Carlson, E.D., Szal, T., Florin, T., Jewett, M.C., and Mankin, A.S. 2015. Protein synthesis by ribosomes with tethered subunits. Nature. 524:119–124.

Areas of Research

cell-free synthetic biology, protein synthesis, therapeutics, glycosylation, engineered ribosomes