Impact of Metallic Nanoparticles on Metal Homeostasis and Use of Synchrotron Techniques to the Study of Silver Nanoparticle Fate in Cells

AbstractThis presentation will review the work that our team has performed on the fate silver nanoparticles in eukaryotic cells. In particular, it will show how x-ray absorption spectroscopy (XAS) can probe the coordination of silver ions in copper biological binding sites, how x-ray fluorescence (XRF) and XAS allows one to visualize, quantify and determine the coordination environment of silver ions released from silver nanoparticles, how labile metal nanoparticles impact cellular homeostasis,how silver nanoparticles interact with metallothionein, and the role of poly-thiols in their dissolution.

BioIsabelle Michaud-Soret received a BS degree in Biochemistry and a PhD in Molecular Pharmacology in 1992 from the University Pierre et Marie Curie - Paris VI - under the supervision of Professor Chottard in the Laboratory of Toxicological and Pharmacological Chemistry and Biochemistry. Her PhD was devoted to mechanistic studies of non-heme iron enzymes, Lipoxygenases. After a post-doctoral position in the laboratory of Professor Que in the Department of Chemistry at the University of Minnesota working on mechanistic and spectroscopic studies of non-heme metalloproteins she obtained a full-time scientist position with the CNRS in Grenoble. In 1994 where she started to work on binuclear manganese metalloproteins before focusing her research on metalloregulators such as the Ferric Uptake regulator (FUR) and NikR. She obtained her Habilitation to direct research in 1999 and became a research director with the CNRS in 2004. Her main scientific interests focus on metalloregulatory proteins able to control gene expression in response to metal ions status in the cell, and the key actors of the metal homeostasis.Since 2010 her research group is also investigating the mechanistic understanding of interferences between metallic nanoparticles and metal homeostasis.