CIV_ENV 447: Molecular Microbiology

Quarter Offered

Winter : MWF 9:00-9:50 AM ; Erica Hartmann


An in-depth look at current molecular methods used to study environmental microbiology. Fundamentals of molecular microbiology, supplemented with discussions of readings from the literature. Creative and critical analysis of study design through proposal writing and reviewing. This course will focus on polymerase chain reaction and derivatives (qPCR, RT-PCR, cloning, CRISPR); DNA sequencing (amplicon sequencing, whole genome sequencing, metagenomics); proteins (proteomics, proteogenomics, metaproteomics); metabolomics; and imaging. Special topics will be considered.

Learning Objectives

By the end of this course, students will be able to:

  • Identify methods of appropriate to research questions
  • Evaluate the application of methods in current research
  • Develop a research proposal wherein methods are explicitly linked to hypothesis


  • 10% Participation
  • 20% Paper discussion
  • 20% Midterms
  • 25% Final Paper: letter of intent, 5-page NSF-style preproposal
  • 25% Proposal Review: participitation in a panel to review peers' proposals


No Formal Text Required


Course Outline, Outcomes, and Goals