Research
Pharmacogenomics

The microarray facility is at the core of the Pharmacogenomics Program. It was built completely from the ground up to create state-of-the-art oligonucleotide microarrays, evaluate the data with full fledged bioinformatics capability, and validate results with quantitative RT-PCR and in situ hybridization techniques.

Equipment

The facility's equipment includes a 96-well PolyPlex® oligonucleotide synthesizer, DNA sequencer, an OmniGrid® robotic spotter for creating the arrays, and a Perkin-Elmer fluorescent scanner using BlueFuse software for spot detection and quantitation. Data are further analyzed using multiple statistical packages followed by detailed ontological analyses using GoMiner® and Gene Set Enrichment Analysis.

DNA Sequencer

High throughput design, synthesis, and optimization of gene-specific 45-mer oligonucleotide.

OmniGrid® Robotic Spotter

In-house robotics for fabrication of microarrays to identify differentially expressed genes with therapeutic potential.

In-House Technology

By having all technologies in house, the Falk Center created a resource for teaching the latest techniques in gene discovery, facilitate collaborations, and provide unique research tools to faculty and graduate students. By the same token, the facility continues to evolve as gene discovery technologies evolve because the microarray program is central to the Falk Center's overall mission, which is to identify novel gene candidates for therapeutic development.

Research Data

qRT-PCR validation of gene expression data

In situ validation of gene expression data

.Multiple statistical algorithms and bioinformatics platforms to identify functionally relevant gene families